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Figure 1.


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(A) Alignment of the EnSerp1 sequence with four of the most similar serpin sequences of other platyhelminths: 1, EnSerp1 from Eudiplozoon nipponicum (GenBank: MF288891.1); 2, Echinococcus multilocularis (GenBank: CDS35969.1); 3, Schistosoma haematobium (GenBank: XP_012797533.1); 4, Echinococcus granulosus (GenBank: CDS22753.1); 5, Taenia solium (GenBank: ATG83400.1). Conserved motifs characteristic for serpins are highlighted. The serpin motif (E342 – E346) shown in red is part of the reactive centre loop (RCL, A347 – N365), shown in green. Immediately after RCL, follows serpin signature (F366 – I376) in yellow. Scissile bond is situated within the RCL between P1 (F358) and P1’ (C359) residue, shown in magenta. (B) Predicted 3D structure of EnSerp1. Coloured areas of the molecule correspond to the sequence highlighted in Figure 1A. (C) RCL and β-sheet A. After the peptidase cleaves the scissile bond within RCL (in orange), the residual part of RCL is incorporated as a new strand into β-sheet A (in cyan).