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Table 2

Prior characterization of P-type transport ATPases in the Trypanosomatidae.

Protein Size (aa) Prior Homologues [Citation] Size (aa) Percent identity Accession Localization (stage) Expression (stage) mRNA
P2A, Ca++ transport (SERCA)
LD2 1023 LDSERCA [154] 1023 94 Q5S054
LMx2 1013 Lmaa1 [87, 137] 1031 87 O09489 ER (P, A) P, A ↑ 3.5 kb
TB2 1011 TBA1 [114, 133] 1011 99 P35315 ER (Pc, B) Pc, B 4.3 kb
TC2s 1006 TcSCA1 [55] 1006 97 O96608 ER (E, T, A) E, T, A 5.0 kb
P2B, Ca++ transport (PMCA)
TB3 1080 TbPMC2 (TbA2) [90] 1080 100 Q8I919 PM (Pc) Pc, B ↑ 4.43 kb
TB4 1106 TbPMC1 (TbA1) [90] 1106 99 Q8I920 AC (Pc) Pc, B ↑ 4.12 kb
TB4b 1102 TBCA2 [154] 340 (p) 96 Q9XZJ8
TC3s/3p 1101/1103 Tca1 [88] 1100 90 O60948 AC, PM (E, T, A) E, T ↑, A ↑↑ 4.3 kb
TCCA2 [154] 341 (p) 98 Q86QH6
P2D, Na+ transport
LD7 1109 LDCA1 [154] 1047 92 O61136 P, A >7.5 kb
TB7 1041 TBCA1 [154] 340 (p) 100 Q9XZJ9 PM (Pc, B) Pc, B ↑ 5.0 kb
TC7s/7p 1039 TcENA [66] 1039 100 Q76DT8 PM (E, T, A) E ↑, T ↑, A 4.5 kb
TCNA1 [154] 340 (p) 97 Q86QH8
P3A, H+ transport
LD8 974 LDH1A [98, 99] 974 97 P11718 P, A ↓ 5.20 kb
LD9 974 LDH1B [98, 99] 974 95 P12522 P, A 5.75 kb
TB8 920 TbHA3 [91] 920 100 Q6WZI6 PM (Pc, B) Pc ↑, B 3.58 kb
TbHA2 [91] 905 92 Q7Z1X2 PM (Pc, B) Pc ↑, B 3.59 kb
TBH1 [154] 257 (p) 100 Q9XZJ7
TB9 912 TbHA1 [91] 912 100 Q86DE0 PM (Pc, B) Pc ↑, B 3.56 kb
TC10s 898 TCH1 [101] 599 (p) 61 O00931
TCH3 [101] 925 64 O15637 E ↑, T 3.8 kb
TcHA1 [89, 170] 875 65 Q8T7V7 PM, R (E, T, A) E ↑, T, A 3.86 kb
TcHA2 [89, 170] 917 64 Q8T7V6 R (E, T, A) E, T ↑, A 5.35 kb
P4, aminophospholipid translocation
LD13 1157 LDAPLT1 [154] 1156 89 O61137
LD15 1097 LdMT [123] 1097 93 Q6VXY9 PM (P)
TC15s/15p 1099 TCAPLT2 [154] 387 (p) 99 Q86QH7

Notes. aa, amino acids; kb, kilobase pairs; PMCA, plasma membrane calcium ATPase; SERCA, sarcoplasmic-endoplasmic reticulum calcium ATPase; (p), partial sequence; AC, acidocalcisome membrane; ER, endoplasmic reticulum membrane; PM, plasma membrane; R, reservosome; A, amastigote stage; B, bloodstream stage trypomastigote; E, epimastigote stage trypomastigote; Pc, procyclic stage trypomastigote; P, promastigote stage; ↑, upregulated; ↓, downregulated. Accession numbers are from the UniProtKB database (http://www.uniprot.org).