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Figure 5

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(A) Expression and purification of UBE2F. (Lane M) protein Mark (ordinate values in kDa); (Lane 1) recombinant UBE2F purified through an Ni-NTA column. (B) Western blot analysis of recombinant UBE2F using DAB. (Lane M) protein Mark (ordinate values in kDa); (Lane 1) recombinant UBE2F recognized by anti-rUBE2F chicken serum; (Lane 2) negative serum control.