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Table 3

Gregarine specimens isolated for molecular investigation.

Host Geographical origin and collection date Number of isolated parasite stages gDNA preparation (name, method) Parasite clones (clone designations)
Locusta migratoria MNHN 2012 Gamonts (50) LW, Phenol chloroform LM1.01.M.2012-1
Locusta migratoria MNHN 2014 Gamonts (50) JF, MasterPure LM2.01.M.2014-2
Locusta migratoria MNHN 2015 Gametocysts (20) JS310, MasterPure LM3.01.M.2015-3
LM3.02.M.2015-4
LM3.03.M.2015-5
LM3.04.M.2015-6
LM3.05.M.2015-7
Locusta migratoria MNHN 2015 Gametocysts (17) JS311, MasterPure LM4.01.M.2015-8
LM4.02.M.2015-9
LM4.03.M.2015-10
LM4.04.M.2015-11
Locusta migratoria MNHN 2015 Gametocysts (13) JS312, MasterPure LM5.01.M.2015-12
LM5.02.M.2015-13
LM5.03.M.2015-14
LM5.04.M.2015-15
Locusta migratoria MNHN 2015 Gametocysts (13) JS313, MasterPure LM6.01.M.2015-16
LM6.02. M.2015-17
LM6.03. M.2015-18
LM6.04. M.2015-19
LM6.05. M.2015-20
Locusta migratoria MNHN 2015 Gametocysts (17) JS314, MasterPure LM7.01. M.2015-21
LM7.02. M.2015-22
LM7.03. M.2015-23
Schistocerca gregaria flaviventris South Africa 2014 Gamonts (10) and Gametocysts (10) JS260, MasterPure SG1.01.SA.2014-24
SG1.02.SA.2014-25
SG1.03.SA.2014-26
SG1.04.SA.2014-27
Schistocerca gregaria flaviventris South Africa 2014 Gametocysts (9) JS261, MasterPure SG2.01.SA.2014-28
SG2.02.SA.2014-29
SG2.03.SA.2014-30
SG2.04.SA.2014-31
SG2.05.SA.2014-32
Schistocerca gregaria flaviventris South Africa 2014 Gamonts (~250) JS269, MasterPure SG3.01.SA.2014-33
SG3.02.SA.2014-34
SG3.03.SA.2014-35
Schistocerca gregaria gregaria Belgium 2014 Gamonts (~200) JS267, MasterPure SG4.01.B.2014-36
SG4.02.B.2014-37
SG4.03.B.2014-38
SG4.04.B.2014-39
SG4.05.B.2014-40
SG4.06.B.2014-41
SG4.07.B.2014-42
Schistocerca gregaria gregaria Morocco 2014 Young trophozoites in ceca (~400) JS272, MasterPure SG5.01.Ma.2014-43