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Levels of IgG (A), sCD4 (B), sCD8 (C), cytokines IFN-γ (D), IL-10 (E), IL-17 (F) and TGF-β1 (G) in chicken sera were measured using ELISA. Chickens of group rEtCHP18905-50μg and group rEtCHP18905-100μg were immunized with 50μg or 100μg of rEtCHP18905 protein, respectively. Challenged and unchallenged groups were immunized with PBS and served as controls. The IgG titers and the concentrations of sCD4, sCD8, and cytokines are expressed as Min to Max. (*p<0.05, **p<0.01, ***p<0.001; ns, p>0.05).